BD FACSAria II is a high-speed cell sorter. It is equipped with a fixed-alignment flow cell cuvette which provides superior fluorescence sensitivity. It can simultaneously detect up to 11 parameters (2 scatter signals and up to 9 fluorescent parameters) by utilizing 3 air-cooled, solid state lasers (laser outputs at 405nm, 488nm and 633nm) and a fixed optical alignment system allowing for multiple configurations and a wide range of fluorochromes. The FACSAria allows for digital acquisition rates of up to 70,000 cells per second. Sorting into different types of test tubes (microtubes, 5 and 15 ml tubes) is possible. The cytometer can sort using Automated Cell Deposition Unit (ACDU) to 96-well plates or on the microscope slide. The sorter is equipped with an Aerosol Management System that evacuates any aerosols from the sort chamber and has a refrigeration system that allows sorting the sample in 4, 21 or 37 degrees.