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  • Flow cytometry
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Analysis and visualization of genes regulatory sequences, using Nencki Genomics Database (human, mouse, rat).
The cytometric method used in the analysis of different intracellular molecules, utilizes intracellular staining protocols. To label intracellular antigens, the cells are fixed and then permeabilized before adding a detection antibody. This fixation/permeabilization procedure allows the ...
Analysis using cationic lipophilic dyes such as JC-1, TMRE (tetramethyl rhodamine ethyl ester), DiOC6 (3), DiIC1 (5), CMXRos (MitoTracker Red), LDS-751, and rhodamine 123, which localize themselves across the inner mitochondrial membrane. Loss of the membrane potential is detected as a decrease ...
Cell cycle analysis is a very popular application of flow cytometry. Utilization of the DNA-specific staining allows you to determine a DNA profile, thus e.g. the percentage of cell populations in phases G0 / G1, S, and G2 / M of a cell cycle. This information may be useful, for example, to ...
Isolation of cells' subpopulations from cell cultures or tissues by FACS (fluorescence-activated cell sorting).
Cell viability is measured as the percentage of living, healthy cells in a population. Cell viability tests are used to determine the general health status of cells, to optimize cell culture or experimental conditions, and to measure cell survival after treatment with tested compounds (e.g. in ...
Critical point drying using CO2. Device used: CPD E3000 Critical Point Dryer (Bio-Rad Polaron Division, UK)
A method of preserving valuable transgenic lines in case of disease outbreak, animal fertility problems, spontaneous loss of phenotype, etc. The full cycle of strain cryopreservation and restoration from frozen material, involves the same biological steps, both for embryo and sperm. The ...
Cytokine detection using bead-based multiplex immunoassays and BD CBA (Cytometric Bead Array) technology as well as BioLegend LEGENDplex kits.
Cytometric method that is suitable for the intracellular signalling pathways analysis. It allows to distinguish different cell types based on surface markers and at the same time enables analysis of signal protein phosphorylation states.
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