Service Category: Flow cytometry

Reactive oxygen species detection

08.09.2020

A method to detect several forms of reactive oxygen species (ROS). Superoxide is detected with OxyBURST Green H2DCFDA or OxyBURST Green H2HFF BSA, and hydrogen peroxide using Carboxy-H2DCFDA and CMH2DCFDA. Detection is possible as a result of the increasing fluorescence of the dye when one of ROS listed above is present in the analysed sample.

Analysis of internal and external membrane proteins (immunocytochemistry labeling)

08.09.2020

The cytometric method used in the analysis of different intracellular molecules, utilizes intracellular staining protocols. To label intracellular antigens, the cells are fixed and then ...

Cytokine detection

08.09.2020

Cytokine detection using bead-based multiplex immunoassays and BD CBA (Cytometric Bead Array) technology as well as BioLegend LEGENDplex kits.

FRET cytometry

08.09.2020

A method of analysis of protein-protein interaction using FRET, a resonance energy transfer phenomenon. The energy transfer takes place between the particles of two fluorochromes with which ...

DNA damage analysis

08.09.2020

High-throughput cytometric detection of the H2AX histone phosphorylation, which is a sensitive marker of DNA damage, especially double-strand breaks (DSBs). It is possible to correlate γH2AX ...