A method of preserving valuable transgenic lines in case of disease outbreak, animal fertility problems, spontaneous loss of phenotype, etc. The full cycle of strain cryopreservation and restoration from frozen material, involves the same biological steps, both for embryo and sperm. The production of fertilized embryos is a key step in the whole process. In the case of cryopreservation of the embryo, the fertilization stage takes place before freezing, and in the case of sperm cryopreservation, after.
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Agata Klejman, PhD